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KMID : 1377020130100060317
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Tissue Engineering and Regenerative Medicine
2013 Volume.10 No. 6 p.317 ~ p.321
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MMP-13 expression in coronal and radicular dentin according to caries progression -a pilot study
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Lee Tae-Yeon
Jin Eun-Jung
Choi Bohm
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Abstract
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Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in degradation of the extracellular matrix under physiologic and pathogenic conditions. In a previous study of the roles of MMPs in dental caries, MMP-13 was found to be expressed in both normal and caries pulp, implying its involvement in the pathogenesis of dental caries. Little is known about functions of MMP-13 related to caries progression. Therefore, the objective of this study was to analyze MMP-13 expression in crown and root of normal and caries dentin by western blot analysis. Seven extracted teeth were used. They were categorized into three grades. (Grade 1: Caries limited to enamel, Grade 2: A wide range of caries that invaded dentin, Grade 3: A wide range of caries that invaded pulp). Teeth were divided into crown and root. MMP-13 expression in extracted protein from separated coronal and radicular dentin was analyzed by western blotting. Western blotting revealed that MMP-13 was present in radicular dentin, and was differently expressed as caries progressed. MMP-13 was not expressed in the coronal dentin groups. MMP-13 expression was increased with caries progression in root dentin. This is the first study to determine the presence of MMP-13 in the root of the dentin. We found that it was distinctly increased in the root dentin of widely progressed caries. Future studies with larger sample sizes may be needed to clearly elucidate changes in MMP-13 expression with progression of caries.
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KEYWORD
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dental caries, dentin, MMP-13
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